The kit is immunochromatographic and uses double-antibody sandwich method to detect SARS-CoV-2 /Influenza A virus /Influenza B virus /Respiratory syncytial virus/Adenovirus antigen. During detection,the treated specimens are loaded into the sample wells of the test card. When the concentration of SARS-CoV-2/Influenza A virus /influenza B virus/Respiratory syncytial virus /Adenovirus antigen in specimen is higher than the minimum detection limit, the viral antigen will form complexes with labeled antibodies first.

Underchromatography, antigen-antibody complexes move forward along the nitrocellulose membrane till captured by precoated monoclonal antibody of SARS-CoV-2/Influenza Avirus/Influenza B virus/Respiratory syncytial virus/Adenovirus in the detection zone on the nitrocellulose film to form a red or blue reaction line on the detection zone indicating the testresult is positive; conversely, if there is no viral antigen or the concentration of antigen inspecimen is below the minimum detection limit, no red/blue reaction line appears in the detection zone, and the test result is negative.

Regardless of whether the sample contains viral antigens or not, a dark blue/purple reaction line will appear in the quality control zone(C). This is the relevant criterion for determining the chromatography process as "normal".